Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study
Identifieur interne : 000138 ( Main/Exploration ); précédent : 000137; suivant : 000139Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study
Auteurs : Bérengère Obstoy [France] ; Mathieu Salaun [France] ; Liana Veresezan [France] ; Richard Sesboüé [France] ; Pierre Bohn [France] ; François-Xavier Boland [France] ; Luc Thiberville [France]Source :
- BMC Pulmonary Medicine [ 1471-2466 ] ; 2015.
English descriptors
- mix :
Abstract
Fibered confocal fluorescence microscopy (FCFM) allows in vivo investigation of pulmonary microstructures. However, the bronchial epithelium can only be imaged using exogenous fluorophores. The objective of this study is to compare methylene blue (MB) and acriflavine genotoxicity and to assess FCFM performance for in vivo imaging of precancerous lesions. Genotoxicity was assessed using the comet assay on both cultured human lymphocytes and NCI-H460 cells, which had been exposed to MB or acriflavine before being illuminated at 660 or 488 nm, respectively. FCFM was performed on precancerous lesions in the hamster cheek pouch model, following topical application of the fluorophores. FCFM data were analyzed according to histology. No genotoxicity was found using 0.01% (w/v) MB after illumination at 660 nm for 2 and 15 min (5 mW). Acriflavine exposure (0.025%) led to DNA damages, increasing from 2 to 15 min of light exposure at 448 nm in both lymphocytes (83.4 to 88%, p = 0.021) and NCI H460 cell populations (79.9 to 84.6%, p = 0.045). In total, 11 invasive carcinoma, 24 reserve cell hyperplasia, and 17 dysplasia lesions were imaged using FCFM in vivo. With both fluorophores, the cellular density increased from hyperplasia to high-grade dysplasia (p < 0.05). With MB, the cellular diameter significantly decreased (48.9 to 13.9 μm) from hyperplasia to carcinoma (p < 0.05). In this model, a cut-off diameter of 30 μm enabled the diagnosis of high-grade lesions with a sensitivity of 94.7% and a specificity of 97%. Methylene blue can be used safely to image precancerous lesions in vivo. This study does not support the use of acriflavine in humans.
Url:
DOI: 10.1186/s12890-015-0020-4
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000285
- to stream Hal, to step Curation: 000285
- to stream Hal, to step Checkpoint: 000080
- to stream Main, to step Merge: 000138
- to stream Main, to step Curation: 000138
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study</title><author><name sortKey="Obstoy, Berengere" sort="Obstoy, Berengere" uniqKey="Obstoy B" first="Bérengère" last="Obstoy">Bérengère Obstoy</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Salaun, Mathieu" sort="Salaun, Mathieu" uniqKey="Salaun M" first="Mathieu" last="Salaun">Mathieu Salaun</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417742" status="VALID"><orgName>CIC Rouen</orgName><desc><address><addrLine>1 rue de Germont - RDC Anneau Central - 76031 Rouen Cedex</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-300106" type="direct"></relation><relation active="#struct-300318" type="direct"></relation><relation name="CIC1404" active="#struct-303623" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300106" type="direct"><org type="institution" xml:id="struct-300106" status="VALID"><orgName>Hôpital Charles Nicolle</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle name="CIC1404" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"><idno type="IdRef">026388278</idno><orgName>Institut National de la Santé et de la Recherche Médicale</orgName><orgName type="acronym">INSERM</orgName><desc><address><addrLine>101, rue de Tolbiac, 75013 Paris </addrLine><country key="FR"></country></address><ref type="url">http://www.inserm.fr</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Veresezan, Liana" sort="Veresezan, Liana" uniqKey="Veresezan L" first="Liana" last="Veresezan">Liana Veresezan</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-301860" status="VALID"> <orgName>CRLCC Henri Becquerel</orgName> <desc> <address> <country key="FR"></country> </address> </desc></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sesboue, Richard" sort="Sesboue, Richard" uniqKey="Sesboue R" first="Richard" last="Sesboüé">Richard Sesboüé</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-182184" status="VALID"><orgName>Génétique du cancer et des maladies neuropsychiatriques</orgName><desc><address><addrLine>UFR Médecine et Pharmacie, 22 Bd Gambetta76183 Rouen Cedex</addrLine><country key="FR"></country></address></desc><listRelation><relation name="U1079" active="#struct-303623" type="direct"></relation><relation active="#struct-300318" type="direct"></relation></listRelation><tutelles><tutelle name="U1079" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"><idno type="IdRef">026388278</idno><orgName>Institut National de la Santé et de la Recherche Médicale</orgName><orgName type="acronym">INSERM</orgName><desc><address><addrLine>101, rue de Tolbiac, 75013 Paris </addrLine><country key="FR"></country></address><ref type="url">http://www.inserm.fr</ref></desc></org></tutelle><tutelle active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Bohn, Pierre" sort="Bohn, Pierre" uniqKey="Bohn P" first="Pierre" last="Bohn">Pierre Bohn</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Boland, Francois Xavier" sort="Boland, Francois Xavier" uniqKey="Boland F" first="François-Xavier" last="Boland">François-Xavier Boland</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-200351" status="VALID"><orgName>Groupe de Recherche sur le Handicap Ventilatoire</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-300147" type="direct"></relation><relation name="EA3830" active="#struct-300318" type="direct"></relation><relation active="#struct-302189" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300147" type="direct"><org type="institution" xml:id="struct-300147" status="VALID"><orgName>CHU Rouen</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA3830" active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-302189" type="direct"><org type="institution" xml:id="struct-302189" status="VALID"><orgName>IRIB Haute-Normandie</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Thiberville, Luc" sort="Thiberville, Luc" uniqKey="Thiberville L" first="Luc" last="Thiberville">Luc Thiberville</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-01153712</idno><idno type="halId">hal-01153712</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-01153712</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-01153712</idno><idno type="doi">10.1186/s12890-015-0020-4</idno><date when="2015">2015</date><idno type="wicri:Area/Hal/Corpus">000285</idno><idno type="wicri:Area/Hal/Curation">000285</idno><idno type="wicri:Area/Hal/Checkpoint">000080</idno><idno type="wicri:doubleKey">1471-2466:2015:Obstoy B:safety:and:performance</idno><idno type="wicri:Area/Main/Merge">000138</idno><idno type="wicri:Area/Main/Curation">000138</idno><idno type="wicri:Area/Main/Exploration">000138</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study</title><author><name sortKey="Obstoy, Berengere" sort="Obstoy, Berengere" uniqKey="Obstoy B" first="Bérengère" last="Obstoy">Bérengère Obstoy</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Salaun, Mathieu" sort="Salaun, Mathieu" uniqKey="Salaun M" first="Mathieu" last="Salaun">Mathieu Salaun</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-417742" status="VALID"><orgName>CIC Rouen</orgName><desc><address><addrLine>1 rue de Germont - RDC Anneau Central - 76031 Rouen Cedex</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-300106" type="direct"></relation><relation active="#struct-300318" type="direct"></relation><relation name="CIC1404" active="#struct-303623" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300106" type="direct"><org type="institution" xml:id="struct-300106" status="VALID"><orgName>Hôpital Charles Nicolle</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle name="CIC1404" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"><idno type="IdRef">026388278</idno><orgName>Institut National de la Santé et de la Recherche Médicale</orgName><orgName type="acronym">INSERM</orgName><desc><address><addrLine>101, rue de Tolbiac, 75013 Paris </addrLine><country key="FR"></country></address><ref type="url">http://www.inserm.fr</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Veresezan, Liana" sort="Veresezan, Liana" uniqKey="Veresezan L" first="Liana" last="Veresezan">Liana Veresezan</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-301860" status="VALID"> <orgName>CRLCC Henri Becquerel</orgName> <desc> <address> <country key="FR"></country> </address> </desc></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Sesboue, Richard" sort="Sesboue, Richard" uniqKey="Sesboue R" first="Richard" last="Sesboüé">Richard Sesboüé</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-182184" status="VALID"><orgName>Génétique du cancer et des maladies neuropsychiatriques</orgName><desc><address><addrLine>UFR Médecine et Pharmacie, 22 Bd Gambetta76183 Rouen Cedex</addrLine><country key="FR"></country></address></desc><listRelation><relation name="U1079" active="#struct-303623" type="direct"></relation><relation active="#struct-300318" type="direct"></relation></listRelation><tutelles><tutelle name="U1079" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"><idno type="IdRef">026388278</idno><orgName>Institut National de la Santé et de la Recherche Médicale</orgName><orgName type="acronym">INSERM</orgName><desc><address><addrLine>101, rue de Tolbiac, 75013 Paris </addrLine><country key="FR"></country></address><ref type="url">http://www.inserm.fr</ref></desc></org></tutelle><tutelle active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Bohn, Pierre" sort="Bohn, Pierre" uniqKey="Bohn P" first="Pierre" last="Bohn">Pierre Bohn</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Boland, Francois Xavier" sort="Boland, Francois Xavier" uniqKey="Boland F" first="François-Xavier" last="Boland">François-Xavier Boland</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-200351" status="VALID"><orgName>Groupe de Recherche sur le Handicap Ventilatoire</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-300147" type="direct"></relation><relation name="EA3830" active="#struct-300318" type="direct"></relation><relation active="#struct-302189" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300147" type="direct"><org type="institution" xml:id="struct-300147" status="VALID"><orgName>CHU Rouen</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA3830" active="#struct-300318" type="direct"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-302189" type="direct"><org type="institution" xml:id="struct-302189" status="VALID"><orgName>IRIB Haute-Normandie</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author><author><name sortKey="Thiberville, Luc" sort="Thiberville, Luc" uniqKey="Thiberville L" first="Luc" last="Thiberville">Luc Thiberville</name><affiliation wicri:level="1"><hal:affiliation type="researchteam" xml:id="struct-426535" status="INCOMING"><orgName>QUANTIF Litis</orgName><desc><address><addrLine>Rouen F-76000</addrLine><country key="FR"></country></address></desc><listRelation><relation active="#struct-23832" type="direct"></relation><relation active="#struct-300317" type="indirect"></relation><relation name="EA4108" active="#struct-300318" type="indirect"></relation><relation active="#struct-301288" type="indirect"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation><tutelles><tutelle active="#struct-23832" type="direct"><org type="laboratory" xml:id="struct-23832" status="VALID"><orgName>Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes</orgName><orgName type="acronym">LITIS</orgName><desc><address><addrLine>Avenue de l'Université UFR des Sciences et Techniques 76800 Saint-Etienne du Rouvray</addrLine><country key="FR"></country></address><ref type="url">http://www.litislab.eu</ref></desc><listRelation><relation active="#struct-300317" type="direct"></relation><relation name="EA4108" active="#struct-300318" type="direct"></relation><relation active="#struct-301288" type="direct"></relation><relation active="#struct-301232" type="indirect"></relation></listRelation></org></tutelle><tutelle active="#struct-300317" type="indirect"><org type="institution" xml:id="struct-300317" status="VALID"><orgName>Université du Havre</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="EA4108" active="#struct-300318" type="indirect"><org type="institution" xml:id="struct-300318" status="VALID"><orgName>Université de Rouen</orgName><desc><address><addrLine> 1 rue Thomas Becket - 76821 Mont-Saint-Aignan</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-rouen.fr/</ref></desc></org></tutelle><tutelle active="#struct-301288" type="indirect"><org type="department" xml:id="struct-301288" status="VALID"><orgName>Institut National des Sciences Appliquées - Rouen</orgName><orgName type="acronym">INSA Rouen</orgName><desc><address><country key="FR"></country></address></desc><listRelation><relation active="#struct-301232" type="direct"></relation></listRelation></org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Le Havre</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université du Havre</orgName><placeName><settlement type="city">Rouen</settlement><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region></placeName><orgName type="university">Université de Rouen</orgName></affiliation></author></analytic><idno type="DOI">10.1186/s12890-015-0020-4</idno><series><title level="j">BMC Pulmonary Medicine</title><idno type="ISSN">1471-2466</idno><imprint><date type="datePub">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Diagnosis</term><term>Fibered confocal fluorescence microscopy</term><term>Genotoxicity</term><term>Heller model</term><term>Methylene blue</term><term>Precancerous lesions</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fibered confocal fluorescence microscopy (FCFM) allows in vivo investigation of pulmonary microstructures. However, the bronchial epithelium can only be imaged using exogenous fluorophores. The objective of this study is to compare methylene blue (MB) and acriflavine genotoxicity and to assess FCFM performance for in vivo imaging of precancerous lesions. Genotoxicity was assessed using the comet assay on both cultured human lymphocytes and NCI-H460 cells, which had been exposed to MB or acriflavine before being illuminated at 660 or 488 nm, respectively. FCFM was performed on precancerous lesions in the hamster cheek pouch model, following topical application of the fluorophores. FCFM data were analyzed according to histology. No genotoxicity was found using 0.01% (w/v) MB after illumination at 660 nm for 2 and 15 min (5 mW). Acriflavine exposure (0.025%) led to DNA damages, increasing from 2 to 15 min of light exposure at 448 nm in both lymphocytes (83.4 to 88%, p = 0.021) and NCI H460 cell populations (79.9 to 84.6%, p = 0.045). In total, 11 invasive carcinoma, 24 reserve cell hyperplasia, and 17 dysplasia lesions were imaged using FCFM in vivo. With both fluorophores, the cellular density increased from hyperplasia to high-grade dysplasia (p < 0.05). With MB, the cellular diameter significantly decreased (48.9 to 13.9 μm) from hyperplasia to carcinoma (p < 0.05). In this model, a cut-off diameter of 30 μm enabled the diagnosis of high-grade lesions with a sensitivity of 94.7% and a specificity of 97%. Methylene blue can be used safely to image precancerous lesions in vivo. This study does not support the use of acriflavine in humans.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li><li>Rouen</li></settlement><orgName><li>Université de Rouen</li><li>Université du Havre</li></orgName></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Obstoy, Berengere" sort="Obstoy, Berengere" uniqKey="Obstoy B" first="Bérengère" last="Obstoy">Bérengère Obstoy</name></region><name sortKey="Bohn, Pierre" sort="Bohn, Pierre" uniqKey="Bohn P" first="Pierre" last="Bohn">Pierre Bohn</name><name sortKey="Boland, Francois Xavier" sort="Boland, Francois Xavier" uniqKey="Boland F" first="François-Xavier" last="Boland">François-Xavier Boland</name><name sortKey="Salaun, Mathieu" sort="Salaun, Mathieu" uniqKey="Salaun M" first="Mathieu" last="Salaun">Mathieu Salaun</name><name sortKey="Sesboue, Richard" sort="Sesboue, Richard" uniqKey="Sesboue R" first="Richard" last="Sesboüé">Richard Sesboüé</name><name sortKey="Thiberville, Luc" sort="Thiberville, Luc" uniqKey="Thiberville L" first="Luc" last="Thiberville">Luc Thiberville</name><name sortKey="Veresezan, Liana" sort="Veresezan, Liana" uniqKey="Veresezan L" first="Liana" last="Veresezan">Liana Veresezan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000138 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000138 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Hal:hal-01153712
|texte= Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study
}}
| This area was generated with Dilib version V0.6.25. |  |